Regulatory

Part:BBa_K2448012

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


pPsiA from Sinorhizobium fredii

This part is the promoter region (0.4 kb upstream) of the PsiA gene of Sinorhizobium fredii (NGR_b11530)

pPsiA is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose [1].

Usage and Biology

Our team used this pPsiA promoter together with the Sinorhizobium fredii PsiR transcription factor (BBa_K2448007) to build a Psicose biosensor (BBa_K2448028). The results show that pPsiA is functional in E. coli and that it behaved as predicted under and without induction. 

Using the BPROM webserver [2], the predicted features of this promoter are:
Threshold for promoters -  0.20
Number of predicted promoters -      1
Promoter Pos:    235 LDF-  1.88
-10 box at pos.    219 cgatagtct Score    37
-35 box at pos.    197 ttgacg    Score    60
Oligonucleotides from known TF binding sites:
     argR2:  GCAAATAA at position     244 Score -   6

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 345
  • 1000
    COMPATIBLE WITH RFC[1000]


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